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QUESTION THREAD3 answers · answered0 views · 16 votes
Transfection efficiency collapses above passage 18 — is this receptor loss or something I am doing?
asked by Alex Rivera (example) · 2026-09-17

Three biological replicates, two reagent lots, fresh medium each time. MC-0288 holds perfectly below p12 and falls apart above p18. Reagent lot is not the variable — S. Bhatt ran L3K-2251 at p10 and got normal numbers. Is this a known receptor-density effect, or am I missing a step?

transfectionHEK293Tpassage number
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GITHUB × STACK OVERFLOW × DATABASE, FOR SCIENTISTS

A failed experiment nobody wrote down gets repeated.

Somewhere else. By someone junior. Next month. SciCollab keeps the data, the code, the conditions and the argument in one place — including the runs that didn't work.

Free for individual researchers · institutional pilots start at one lab
debug(research) → knowledge
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FOUR THINGS, ORGANISED
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Artifact-rich questions

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Structured metadata

Method, conditions, outcomes — captured as a first-class object, not a paragraph in a PDF.

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FIRST-CLASS OBJECT
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